Plants must continuously balance growth with stress responses. In Arabidopsis thaliana, we found that the transcription factor NLP7, a central regulator of nitrogen signaling, also
represses drought- and ABA-responsive transcription factors, integrating antagonistic environmental cues. Using ChIP-seq and the cell-based TARGET system, we identified
direct NLP7 targets and their regulatory roles. nlp7 mutants exhibit enhanced drought tolerance, even under glutamine-based nutrition, revealing that drought resilience can
occur independently of growth impairment. We extended this approach to tomato (Solanum lycopersicum), where we performed leaf transcriptome profiling under combined nitrogen and drought treatments. This analysis uncovered genes specifically regulated by the interaction of both signals. To study relevant TFs activity at genome scale, we established DAP-seq and TARGET platforms in tomato. (...)