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SUMMARY:Pflanzenwissenschaftliches Kolloquium
DTSTART;TZID=Europe/Berlin:20260123T120000
DTEND;TZID=Europe/Berlin:20260123T130000
DTSTAMP:20260915T214400Z
UID:4fdd1cd9e8964c9989059f2d50baaaef@www.biologie.uni-bonn.de
CREATED:20251127T130439Z
DESCRIPTION:The expansion of gene families via duplication is a characteri
 stic feature of plant genomes and presents a major challenge for assigning
  biological functions using single-gene knockouts. Here\, we present a str
 ategy for analyzing the biological functions of highly expanded enzyme fam
 ilies in A. thaliana\, using the cytochrome P450 subfamily 71B as an examp
 le. This subfamily contains 32 expressed genes\, and only a few are functi
 onally characterized\, such as CYP71B15 (PAD3)\, an essential component of
  a camalexin biosynthetic complex. Aiming for generation of a complete 32x
  knockout\, we have deleted four CYP71B clusters (up to 83 kb in size) usi
 ng CRISPR/Cas9-based gene editing. Metabolite compositions are being syste
 matically analyzed via UHPLC/Q-TOF-MS suggesting functions contributing to
  defense and/or communication. Here\, a cyp71b11-cyp71b13 deletion mutant 
 is impaired in the formation of hydroxycamalexin glycosides\, which result
 s in enhanced camalexin autotoxicity.
LAST-MODIFIED:20251127T130500Z
URL:https://www.biologie.uni-bonn.de/de/veranstaltungen/pflanzenwissenscha
 ftliches-kolloquium-10
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